A

Protein synthesis (translation) in eukaryotes requires the ribosome with its peptidyl-transferase activity and charged tRNAs produced by amino-acyl tRNA synthetases; RNA polymerase is needed earlier for transcription of mRNA but plays no direct role in the translational machinery itself [Rodnina, 2013, PMID 23711800; Griesenbeck et al., 2017, PMID 28271479].