MAINMAST is a filly automated protocol and can generate reliable initial C-alpha models which can be used to construct full atomic models. This new de novo modeling method has several advantages; (1) It does not require reference structures; (2) It does not requre manual interventions; (3) a pool of candidate models are generated.
(1) Identify local dense points (LDPs) by Mean Shifting Algorithm;
(2) Connect all LDPs by Minimum Spanning Tree;
(3) Refine Tree structure by Tabu Search algorithm;
(4) Thread sequence on the longest path.